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Open Access Research

Correlation of tumor-associated macrophages and clinicopathological factors in Wilms tumor

Peter Liou1, Leah Bader1, Antai Wang3, Darrell Yamashiro2 and Jessica J Kandel1*

Author Affiliations

1 Division of Pediatric Surgery, Columbia University Medical Center, 3959 Broadway, CHN 214, New York, NY, 10032, USA

2 Division of Pediatric Hematology/Oncology/Stem Cell Transplantation, Columbia University Medical Center, New York, NY, 10032, USA

3 Department of Biostatistics, Columbia University Mailman School of Public Health, New York, NY, 10032, USA

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Vascular Cell 2013, 5:5  doi:10.1186/2045-824X-5-5

Published: 21 March 2013

Abstract

Background/purpose

Despite high long-term survival rates in patients with Wilms tumor, there is a need to develop better prognostic biomarkers in order to maximize cure while avoiding treatment-associated morbidities. Tumor-associated macrophages have been recently associated with poorer prognosis and increased disease progression in a number of adult cancers. We investigated the relationship between macrophages and clinicopathological fators in this pediatric solid tumor.

Methods

Tissue microarray sections of 124 Wilms tumor cases obtained from the Cooperative Human Tissue Network were stained with CD68, a macrophage marker using standard immunohistochemical techniques and quantified using digital image processing techniques. Macrophage densities were correlated by tumor stage, and survival analyses were conducted with available clinical data. Immunohistochemistry was performed on 25 additional Wilms tumor cases obtained from the tumor bank at Columbia University Medical Center and correlated with presence of tumor microvascular invasion.

Results

Mean macrophage count densities in stage IV tumors were significantly greater than densities in stage I and III tumors (p=.021, .036). Although the overall and disease-free survival did not differ between high and low macrophage presence groups across all stages, increased macrophage presence was associated with decreased disease-free survival in patients with stage II tumors (p=0.035). Increased macrophage presence may have also correlated with decreased disease-free survival in stage IV patients, but the sample size was too small to allow detection of this difference with significance (p=0.575). Increased macrophage presence was associated with tumor microvascular invasion (p=0.0004).

Conclusion

Our results suggest that macrophage recruitment may be associated with disease progression in Wilms tumor. Quantitation of macrophage presence may therefore be a useful adjunct in refining staging algorithms for patients with stage II Wilms tumor. Such data might be useful in the effort to reduce the risk of adverse effects associated with under- or overtreatment of this neoplasm.

Keywords:
Wilms tumor; Tumor-associated macrophages; Wilms tumor staging; Lymph node sampling