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Open Access Correction

Correction: Co-culture of Retinal and Endothelial Cells Results in the Modulation of Genes Critical to Retinal Neovascularization

Ravindra Kumar1*, Sandra Harris-Hooker2, Ritesh Kumar3 and Gary Sanford1

Author Affiliations

1 Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, 720 Westview Drive, S.W., Atlanta, Georgia 30310, USA

2 Department of Pathology, Morehouse School of Medicine, 720 Westview Drive, S.W., Atlanta, Georgia 30310, USA

3 Undergraduate student, Department of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30322, USA

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Vascular Cell 2012, 4:6  doi:10.1186/2045-824X-4-6


The electronic version of this article is the complete one and can be found online at: http://www.vascularcell.com/content/4/1/6


Received:14 March 2012
Accepted:26 March 2012
Published:26 March 2012

© 2012 Kumar et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Correction

Following publication of our article [1] it was noted that Figures five E and five G were the same as Figures six A and six B. Figure 1 in this correction article is the correct version of Figure six that should have been included in the original article [1]. We apologize for any inconvenience caused by this error.

thumbnailFigure 1. RT-PCR and Western blot analysis of NFκB. Total RNA and total protein were extracted from HRPC and HUVEC cultured alone or co-cultured under normoxia and hypoxia conditioned for 24 h. The expression of NFκB was measured by (A) electrophoresis of RT-PCR, (C) Western blot analysis in the HRPC and HUVEC. Figures (B, D) the band intensities corresponding to the NFκB were quantified by image analysis using a Bio-Rad scanning densitometer and Quantity One analysis software. Data were shown as ratio of NFκB densities to that of 18S RNA for RT-PCR and β-actin antibody was used to normalize Western blot for differences in loading and the transfer efficiencies. All data were expressed as mean +/- SE and results are representatives of three independent experiments.

References

  1. Kumar R, Harris-Hooker S, Kumar R, Sanford G: Co-culture of Retinal and Endothelial Cells Results in the Modulation of Genes Critical to Retinal Neovascularization.

    Vascular Cell 2011, 3:27. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL