Research
Lymphoid enhancer-binding factor 1, a representative of vertebrate-specific Lef1/Tcf1 sub-family, is a Wnt-beta-catenin pathway target gene in human endothelial cells which regulates matrix metalloproteinase-2 expression and promotes endothelial cell invasion
-
* Corresponding author: Marina Planutiene marina.planoutene@mssm.edu
- † Equal contributors
The Tisch Cancer Institute at The Mount Sinai Medical Center, One Gustave L Levy Place, New York, NY 10029-6500, USA
Vascular Cell 2011, 3:28 doi:10.1186/2045-824X-3-28
Published: 14 December 2011Additional files
Additional file 1:
Effect of Lef1 siRNA on Lef1 mRNA in the absence of Wnt3a. Full length Lef1 mRNA levels by qRT-PCR in EAhy926 cells treated by control CM (without Wnt3a) in the presence of control RNA (column 1) and Lef1 siRNA (columns 3 and 4). The amount of Lef1 mRNA is reduced in cells treated with siRNA even without stimulation by Wnt3a (**p = 0.0018).
Format: PPTX Size: 69KB Download file
Additional file 2:
Effect of Lef1 expression on MMP1 mRNA. MMP1 mRNA levels measured by qRT-PCR in EAhy926 cells. Transfection with a full-length Lef1 expression construct does not affect significantly the amount of MMP1 mRNA.
Format: PPTX Size: 79KB Download file
Additional file 3:
Prolonged proliferation of EAhy926 cells measured by XTT assay. Transfection with a full-length Lef1 expression construct slightly increases proliferation of EAhy926 cells after 120 hours (**p = 0.0077). Here we used the TACS® XTT Cell Proliferation Assay from R & D Systems.
Format: PPTX Size: 81KB Download file