Figure 4.

Macrophage recruitment is altered by BV and SC236 treatment. Distribution of macrophages was examined using the mouse macrophage marker F4/80 (Figure 4A). Morphologically, F4/80 appeared to be increased in BV- and BV+SC236-treated tumors, with somewhat more irregularity in the latter. Quantified F4/80(+) signal was significantly increased in BV- and SC236+BV-treated groups versus controls (Figure 4B). TNFα-immunopositive cell distribution appeared similar to the patterns for F4/80 signal (Figure 4C). The M2 marker arginase was detected in cells primarily in necrotic regions, which did not change with treatment (not shown).