Open Access Methodology

Effects on duration of post-operative ischemia and patterns of blood flow recovery in different conditions of mouse hind limb ischemia

Husain A Al-Mubarak12, Talal M Alamri12, Saif A Aljabab12, Mohammad Atteya1, Adrian Quan2, Hwee Teoh2, Praphulla C Shukla2, Subodh Verma2, Abdullah Aldahmash12, Badr Aljabri12, Claudio Napoli13 and Mohammed Al-Omran12*

Author Affiliations

1 Peripheral Vascular Disease Research Chair and The Stem Cell Unit, College of Medicine, King Saud University, Riyadh, Saudi Arabia

2 King Saud University-Li Ka Shing Knowledge Institute of St Michael's Hospital Collaborative Research Program, Toronto, Canada

3 Department of General Pathology, Division of Clinical Pathology and Excellence Research Center on Cardiovascular Diseases, 1st School of Medicine, II University of Naples, 80138 Naples, Italy

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Vascular Cell 2011, 3:14  doi:10.1186/2045-824X-3-14

Published: 14 June 2011

Abstract

Background

Current limitations to the experimentation on patients with peripheral arterial disease push the development of different preclinical strategies. We investigated both duration of ischemia and blood flow recovery in mouse models of partial femoral artery ligation.

Methods

Male BALB/c mice were used. The ligation over needle method involved placing a suture needle over the femoral artery, ligating over it and then removing the needle. The transfixation method involved transfixing the approximate center of the femoral artery and then tying the suture. Laser Doppler Perfusion Imaging was used to assess perfusion every 3rd day until 42 days after the procedure.

Results

Ligation over needle method: Immediately post procedure, mean perfusion was -71.87% ± 4.43. Then mean difference in perfusion remained below the base line reading on days 3, 6, 9, and 12. From day 15 on wards mean perfusion progressively improved remaining near base line. Transfixation Method: Immediately post procedure mean perfusion was -70.82% ± 4.73. Mean perfusion improved following the procedure on days 3 and 6; a plateau followed this on days 9, 12 and 15. From day 15 onwards perfusion progressively improved remaining well below base line until crossing it on day 36.

Conclusion

The currently described models do not pose major improvements over previously described methods.

Keywords:
Hindlimb Ischemia; Angiogenesis; LDPI; Mouse Model; Peripheral Arterial Disease