Research
Tumor growth and angiogenesis is impaired in CIB1 knockout mice
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* Corresponding author: Leslie V Parise parise@med.unc.edu
1 Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
2 Department of Biochemistry & Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
3 Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
4 Current Address: Department of Surgery, Division of Vascular Surgery, Stanford University Hospital & Clinics, Stanford, CA, USA
5 Current Address: Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin, China
Journal of Angiogenesis Research 2010, 2:17 doi:10.1186/2040-2384-2-17
Published: 30 August 2010Additional files
Additional file 1:
Carcinoma tumors in CIB1-KO mice have less areas of high perfusion. Carcinoma tumors in CIB1-KO mice have reduced perfusion. Superficial hind-limb ventral adductor thigh regions, where tumor cells were injected, were monitored with noninvasive scanning laser-Doppler perfusion imaging (model LD12-IR, Moor Instruments; modified for high resolution and depth of penetration (2 mm) with an 830 nm-wavelenght infrared 2.5 mW laser diode, 100 μm beam diameter, and 15 kHz bandwidth). Isofluorane anesthesia and rectal temperature (37 ± 0.5°C) were maintained the same for all measurement days and among animals. (a) Laser-Doppler perfusion imaging at the site of tumor injection pre- (day 1), and day-7 and -14 after tumor cell injection. Image scans are assembled from the mean Doppler velocity (in perfusion units, PU) for each 100 × 2000 μm voxel in the scan area. (b) Regions of interest (ROI) were drawn around tumor perimeters, to derive a tumor area, and determine what percentage of the area had a detectable Doppler signal. For every given ROI, a tumor perfusion index was calculated using: (Average PU) × (ROI area) × (% ROI with detectable Doppler signal)/10,000. Error bars are ± SEM (n = 8-9 mice per group).
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