Figure 7.

Time-series visualization of a developing intravascular pillar using fluorescence intravital videomicroscopy. Image stacks (100 images) of the CAM vessels plasma marker FITC-dextran were obtained at t = 0 and t = 38 minutes. A) The initial image stack revealed 3 intravascular pillars (arrows). B) At t = 38 minutes, a new intravascular pillar was identified (large arrow) accompanied by notable membrane irregularity (small arrow). C) Computational flow analysis of the vessel bifurcation without pillars demonstrated an area of low shear stress at the site of the initial pillar (arrow). D) Morphometry of the initial (A) and subsequent (B) vessels showed a significant increase in pillar area (hole area) and membrane irregularity (perimeter). Bar = 50 um.